Hauf Research Group

Hauf Research Group

We investigate how cells robustly execute cell division, a process that is highly dynamic and requires intricate regulation. The group combines quantitative perturbations, live cell imaging, and computational modeling to understand the underlying regulation.

The separation of the chromosomes in anaphase is one of the most dramatic events of the cell cycle. It is irreversible and therefore needs to be highly accurate and tightly coordinated with mitotic exit. We combine perturbation experiments, live cell imaging, and computational modeling to understand how this high degree of coordination is achieved.

Related Publication

Slow checkpoint activation kinetics as a safety device in anaphase. Curr Biol, 2014.

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When cells divide, they need to pass on copies of the genetic information to both daughter cells. This step is controlled by a signaling pathway called the spindle assembly checkpoint. If the checkpoint fails, cells can become aneuploid, i.e., have the wrong number of chromosomes, which is a condition associated with cancer. We study how the checkpoint signaling network is constructed and which features allow it to work reliably.

Related Publications

Determinants of robustness in spindle assembly checkpoint signalling. Nat Cell Biol, 2013.

Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores. EMBO Rep, 2014.

Lipid-binding Events in the Wnt Signaling Pathway

Our aim is to understand basic conserved principles of cellular regulation and to be able to quantitatively explain cellular phenomena. In most of our work, we therefore combine experiments with computational modeling. This allows us to explore types of regulation that are too difficult to understand intuitively. We work with fission yeast, a simple eukaryote, which is an excellent model for eukaryotic cells in general, including human cells.

Modulators of Platelet Aggregation

Lab Members

Name
Title

Baybay, Erod

Research Assistant

Boluarte, Tatiana

Lab manager

Gopala Krishna, Varun

Postdoctoral researcher

Morton, Claire

High school student

Pribadi, Joshua

Undergraduate student researcher

Rogers, Jessie

Graduate student

Vijayakumari, Drisya

Postdoctoral researcher

Weidemann, Douglas

Lab specialist

Williams, Wendi

Undergraduate student researcher

Woodhouse, Mitchell

Undergraduate student researcher

2019

Barbosa AC, Xu Z, Karari K, Hauf S, Brown WR. Budding yeast centromeric DNA and A+T rich bacterial DNA can function as centromeres in the fission yeast Schizosaccharomyces pombe. [preprint]. 2019 January; doi: 10.1101/513150.

2018

Gross F, Bonaiuti P, Hauf S, Ciliberto A. Implications of alternative routes to APC/C inhibition by the mitotic checkpoint complex. PLoS Comput Biol. 2018 Sep 10;14(9):e1006449. doi: 10.1371/journal.pcbi.1006449. PubMed PMID: 30199529.

2017

Maček B, Carpy A, Koch A, Bicho CC, Borek WE, Hauf S, Sawin KE. Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technology in Fission Yeast. Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.top079814. doi: 10.1101/pdb.top079814. PubMed PMID: 28572211.

Carpy A, Koch A, Bicho CC, Borek WE, Hauf S, Sawin KE, Maček B. Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Quantitative Proteomics and Phosphoproteomics in Fission Yeast. Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.prot091686. doi: 10.1101/pdb.prot091686. PubMed PMID: 28572185.

Koch A, Bicho CC, Borek WE, Carpy A, Maček B, Hauf S, Sawin KE. Construction, Growth, and Harvesting of Fission Yeast Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Strains. Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.prot091678. doi: 10.1101/pdb.prot091678. PubMed PMID: 28572184.

Sewart K, Hauf S. Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex. Curr Biol. 2017 Apr 24;27(8):1213-1220. doi: 10.1016/j.cub.2017.03.007. Epub 2017 Mar 30. PubMed PMID: 28366743.

Ciliberto A, Hauf S. Micromanaging checkpoint proteins. Elife. 2017 Feb 16;6. pii: e25001. doi: 10.7554/eLife.25001. PubMed PMID: 28206949.

2016

Kamenz J, Hauf S. Time To Split Up: Dynamics of Chromosome Separation. Trends Cell Biol. 2017 Jan;27(1):42-54. doi: 10.1016/j.tcb.2016.07.008. Epub 2016 Aug 24. Review. PubMed PMID: 27567180.

Geissen EM, Hasenauer J, Heinrich S, Hauf S, Theis FJ, Radde NE. MEMO: multi-experiment mixture model analysis of censored data. Bioinformatics. 2016 Aug 15;32(16):2464-72. doi: 10.1093/bioinformatics/btw190. Epub 2016 Apr 19. PubMed PMID: 27153627.

2015

Kamenz J, Mihaljev T, Kubis A, Legewie S, Hauf S. Robust Ordering of Anaphase Events by Adaptive Thresholds and Competing Degradation Pathways. Mol Cell. 2015 Nov 5;60(3):446-59. doi: 10.1016/j.molcel.2015.09.022. Epub 2015 Oct 29. PubMed PMID: 26527280.

2014

Carpy A, Krug K, Graf S, Koch A, Popic S, Hauf S, Macek B. Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast). Mol Cell Proteomics. 2014 Aug;13(8):1925-36. doi: 10.1074/mcp.M113.035824. Epub 2014 Apr 23. PubMed PMID: 24763107.

Kamenz J, Hauf S. Slow checkpoint activation kinetics as a safety device in anaphase. Curr Biol. 2014 Mar 17;24(6):646-51. doi: 10.1016/j.cub.2014.02.005. Epub 2014 Feb 27. PubMed PMID: 24583014.

Heinrich S, Sewart K, Windecker H, Langegger M, Schmidt N, Hustedt N, Hauf S. Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores. EMBO Rep. 2014 Mar;15(3):291-8. doi: 10.1002/embr.201338114. Epub 2014 Jan 29. PubMed PMID: 24477934.